発表論文 (原著論文、著書、総説など)

2016 2015 2014 2013 2012 2011 2010 2009 2008 2007
2006年以前

2016

  • Kaboli, S., Miyamoto, T., Sunada, K., Sasano, Y., Sugiyama, M., Harashima, S.: Improved stress resistance and ethanol production by segmental haploidization of the diploid genome in Saccharomyces cerevisiae. J. Biosci. Bioeng., 121: 638-644 (2016).
  • Sugiyama, M., Akase, S., Nakanishi, R., Kaneko, Y., Harashima, S.: Overexpression of ESBP6 improves lactic acid resistance and production in Saccharomyces cerevisiae. J. Biosci. Bioeng., 122: 415-420 (2016).
  • Sasano, Y., Nagasawa. K., Kaboli. S., Sugiyama, M., Harashima, S.: CRISPR-PCS: a powerful new approach to inducing multiple chromosome splitting in Saccharomyces cerevisiae. Scientific Reports, 17: 30278 (2016).
  • Kitichantaropas, Y., Boonchird C., Sugiyama, M., Kaneko, Y., Harashima, S.,and Auesukaree, C.: Cellular mechanisms contributing to multiple stress tolerance in Saccharomyces cerevisiae strains with potential use in high-temperature ethanol fermentation. AMB Express, 6: 107 (2016).
  • 杉山峰崇,笹野 佑,鈴木 俊宏,原島 俊: 高温・有機酸ストレス耐性出芽酵母の育種と発酵生産への利用. 化学と生物, 54: 820-826 (2016).

2015

  • Sasano, Y., Yamagishi, K., Tanikawa, M., Nakazawa, T., Sugiyama, M., Kaneko, Y., Harashima, S.: Stabilization of mini-chromosome segregation during mitotic growth by overexpression of YCR041W and its application to chromosome engineering in Saccharomyces cerevisiae. J. Biosci. Bioeng., 119: 526-531 (2015).
  • Numamoto, M., Tagami, S., Ueda, Y., Imabeppu, U., Sasano, Y, Hirasaki, M., Sugiyama, M., Maekawa, H., Harashima, S.: Nuclear localization domains of GATA activator Gln3 are required for transcription of target genes through dephosphorylation in Saccharomyces cerevisiae. J. Biosci. Bioeng., 120: 121-127 (2015).
  • Natesuntorn, W., Iwami, K., Matsubara, Y., Sasano, Y., Sugiyama, M., Kaneko,Y., Harashima, S.: Genome-wide construction of a series of designed segmental aneuploids in Saccharomyces cerevisiae. Scientific Reports, 5: 12510 (2015).
  • Hermansyah, Novia, Sugiyama, M., Harashima, S.: Candida tropicalis isolated from Tuak, a north sumatera-indonesian traditional beverage, for bioethanol production. Microbiol. Biotechnol. Lett., 43: 241-248 (2015).
  • Sugiyama, M., Sasano, Y. and Harashima, S.: Adaptation mechanism of yeast to weak organic acid. In Kitagaki, H. and Takagi, H. (Eds.), Stress Biology of Yeasts and Fungi: Application for Industrial Brewing and Fermentation. Springer Japan. p. 107-121 (2015).
  • 新聞報道:日本経済新聞 朝刊「古紙や間伐材からバイオ燃料」 杉山峰崇 2015年6月1日.

2014

  • 笹野 佑、Yeon-Hee Kim、杉山 峰崇、原島 俊  多様性創出ゲノム工学技術の開発と微生物育種への応用、生物工学会誌、第92巻、第11号、 589-592 (2014)
  • Sasano, Y., Yamagishi, K., Tanikawa, M., Nakazawa, T., Sugiyama, M., Kaneko, Y., Harashima, S. (2014) Stabilization of mini-chromosome segregation during mitotic growth by overexpression of YCR041W and its application to chromosome engineering in Saccharomyces cerevisiae. J. Biosci. Bioeng. Nov:15(14)1389-1723
  • Sharmin, D., Sasano, Y., Sugiyama, M., Harashima, S. (2014) Effects of deletion of different PP2C protein phosphatase genes on stress responses in Saccharomyces cerevisiae. Yeast Oct: 31(10):393-409.
  • Kaboli, S., Yamakawa, T., Sunada, K., Takagaki, T., Sasano, Y., Sugiyama, M., Kaneko, Y., Harashima, S. (2014) Genome-wide mapping of unexplored essential regions in the Saccharomyces cerevisiae genome: evidence for hidden synthetic lethal combinations in a genetic interaction network. Nucleic Acids Res. Aug: 42(15):9838-53.
  • Sugiyama, M., Akase, SP., Nakanishi, R., Horie, H., Kaneko, Y., Harashima, S. (2014) Nuclear localization of Haa1, which is linked to its phosphorylation status, mediates lactic acid tolerance in Saccharomyces cerevisiae. Appl. Environ. Microbiol. Jun: 80(11):3488-95.
  • Lavina, W., Shahsavarani, H., Saidi, A., Sugiyama, M., Kaneko, Y., Harashima, S. (2014) Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1. J. Biosci. Bioeng. Feb: 117(2):135-41.
  • Sangwallek, J., Kaneko, Y., Tsukamoto, T., Marui, M., Sugiyama, M., Ono, H., Bamba, T., Fukusaki, E., Harashima, S. (2014) Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha. Gene. Jan: 533(1):110-8.
  • 原島 俊  Yeast: A Living Tool for Biotechnology(Chapter 1)、実践・化学英語リスニング、福井 希一・福住 俊一・ルーク 上田 サーソン編  (株)化学同人、3-8
  • Sasano Y, Sugiyama M. Harashima S. (2014) Chapter5: Development and application of novel genome engineering technology in Saccharomyces cerevisiae. Microbial production -From genome design to cell engineering-, Microbial Production Anazawa H and Simizu S eds., Springer, 53-62.

2013

  • Shahsavarani, H., Hasegawa, D., Yokota, D., Sugiyama, M., Kaneko, Y., Boonchird, C., Harashima, S. (2013) Enhanced bio-ethanol production from cellulosic materials by semi-simultaneous saccharification and fermentation using high temperature resistant Saccharomyces cerevisiae TJ14. J. Biosci. Bioeng. Jan: 115(1): 20-3
  • Lavina, W. A., Hermansyah, Sugiyama, M., Kaneko, Y., Harashima, S. (2013) Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration. J. Biosci. Bioeng. Feb: 115(2):138-46.
  • 原島 俊  合成生物工学の時代と科学技術のデュアルユース問題、バイオサイエンスとインダストリー、Vol.71、No1、 6-7 (2013)
  • 杉山 峰崇、笹野 佑、原島 俊  酵母ゲノム工学の最前線、細胞工学、Vol.32、No5、 592-599 (2013)
  • Suzuki, T., Sakamoto, T., Sugiyama, M., Ishida, N., Kambe, H., Obata, S., Kaneko, Y., Takahashi, H., Harashima, S. (2013) Disruption of multiple genes whose deletion causes lactic-acid resistance improves lactic-acid resistance and productivity in Saccharomyces cerevisiae. J. Biosci. Bioeng. May: 115(5):467-74.
  • Sooksai, S., Chewchanlertfa, P., Kaneko, Y., Harashima, S., Laoteng, K. (2013) Alterations in growth and fatty acid profiles under stress conditions of Hansenula polymorpha defective in polyunsaturated fatty acid synthesis. Mol. Biol. Rep. Aug:40(8):4935-45.
  • Sasano, Y., Haitani, Y., Hashida, K., Oshiro, S., Shima, J. and Takagi, H. (2013) Improvement of fermentation ability under baking-associated stress conditions by altering the POG1 gene expression in baker`s yeast. Int. J. Food Microbiol. Aug:165(3): 241-45.
  • 原島 俊  RNAポリメラーゼIによる転写制御(第2章)・RNAポリメラーゼUによる転写制御(第3章)・pHストレス(酸・アルカリ) (第12章)、酵母の生命科学と生物工学、原島 俊・高木 博史編  (株)化学同人、17-30、31-50、183-196 (2013)
  • Khatun, F., Kurata, K., Chuwattanakul, V., Sugiyama, M., Kaneko, Y., Harashima, S. (2013) Increased transcription of RPL40A and RPL40B is important for the improvement of RNA production in Saccharomyces cerevisiae. J. Biosci. Bioeng. Oct:116(4):423-32.
  • Khatun, F., Sasano, Y., Sugiyama, M., Kaneko, Y., Harashima, S. (2013) Increase in rRNA content in a Saccharomyces cerevisiae suppressor strain from rrn10 disruptant by rDNA cluster duplication. Appl. Microbiol. Biotechnol. Oct: 97(20):9011-9.
  • Sangwallek, J., Kaneko, Y., Sugiyama, M., Ono, H., Bamba, T., Fukusaki, E., Harashima, S. (2013) Ketoacyl synthase domain is a major determinant for fatty acyl chain length in Saccharomyces cerevisiae. Arch. Microbiol. Dec:195(12):843-52.
  • Lavina, W., Shahsavarani, H., Saidi, A., Sugiyama, M., Kaneko, Y., Harashima, S. (2014) Suppression mechanism of the calcium sensitivity in Saccharomyces cerevisiae ptp2Δmsg5Δ double disruptant involves a novel HOG-independent function of Ssk2, transcription factor Msn2 and the protein kinase A component Bcy1. J. Biosci. Bioeng. Feb: 117(2):135-41.
  • Sangwallek, J., Kaneko, Y., Tsukamoto, T., Marui, M., Sugiyama, M., Ono, H., Bamba, T., Fukusaki, E., Harashima, S. (2014) Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha. Gene. Jan: 533(1):110-8.
  • 笹野 佑  最新のゲノム編集技術が拓く新たな微生物育種への道、生物工学会誌、Vol.91、No12、 719 (2013)

2012

  • Ueda, Y., Ikushima, S., Sugiyama, M., Matoba, R., Kaneko, Y., Matsubara, K., Harashima, S. (2012) Large-scale genome reorganization in Saccharomyces cerevisiae through combinatorial loss of mini-chromosomes. J. Biosci. Bioeng. Jun : 113(6):675-82
  • Dwiarti, L., Boonchird, C., Harashima, S., Park, Y., E., (2012) Simultaneous saccharification and fermentation of paper sludge without pretreatment using cellulase from  Acremonium cellulolyticus and thermotolerant Saccharomyces cerevisiae. Biomass and Bioenergy July 42: 114-122
  • Chuwattanakul, V., Sugiyama, M., Khatun, F., Kurata, K., Tomita, I., Kaneko, Y., Harashima, S. (2012) Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae , enhances the production yield of RNA as a source of nucleotide seasoning. J. Biosci. Bioeng. Jul;114(1): 17-22
  • Auesukaree, C., Koedrith, P., Saenpayavai, P., Asvarak, T., Benjaphokee, S., Sugiyama, M., Kaneko, Y., Harashima, S., Boonchird, C. (2012) Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. J. Biosci. Bioeng. Aug;114(2): 144-9
  • Park, AH., Sugiyama, M., Harashima, S., Kim, YH. (2012) Creation of an ethanol-tolerant yeast strain by genome reconstruction based on chromosome splitting technology. J. Microbiol. Biotechnol. Feb:22(2): 184-9
  • Shahsavarani, H., Sugiyama, M., Kaneko, Y., Chuenchit, B., Harashima, S. (2012) Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase. Biotechnol. Adv. Nov:30(6): 1289-300
  • 原島 俊・Yeon-Hee Kim・西沢正文  出芽酵母におけるゲノムの大規模改変技術の開発と応用 微生物を活用した新世代の有用物質生産技術、穴澤秀治監修、(株)シーエムシー出版、32-41 (2012)
  • 原島 俊・西沢正文  出芽酵母ゲノムの自在な操作と細胞育種への応用 合成生物工学の隆起―有用物質の新たな生産法構築をめざして―、植田充美監修、(株)シーエムシー出版、10-17 (2012)
  • Benjaphokee, S., Hasegawa, D., Yokota, D., Asvarak, T., Auesukaree, C., Sugiyama, M., Kaneko, Y., Boonchird, C., Harashima, S. (2012) Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol. N. Biotechnol. Feb 15;29(3): 379-86
  • Benjaphokee, S., Koedrith, P, Auesukaree C., Asvarak, T., Sugiyama, M., Kaneko, Y., Boonchird, C., Harashima, S. (2012) CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae. New Biotechnology 29(2):166-76.
  • Suzuki, T., Sugiyama, M., Wakazono, K., Kaneko, Y., Harashima, S. (2012) Lactic-acid stress causes vacuolar fragmentation and impairs intracellular amino-acid homeostasis in Saccharomyces cerevisiae. J. Biosci. Bioeng. 113(4):421-30.
  • Sasano, Y., Haitani, Y., Ohtsu, I., Shima, J. and Takagi H. (2012) Proline accumulation in baker’s yeast enhances high-sucrose stress tolerance and fermentation ability in sweet dough. Int. J. Food Microbiol. 152: 40-43 
  • Sasano, Y., Haitani, Y., Hashida, K., Ohtsu, I., Shima, J. and Takagi, H. (2012) Overexpression of the transcription activator Msn2 enhances fermentation ability of industrial baker’s yeast in frozen dough. Biosci. Biotech. Biochem. 76: 624-627
  • Sasano, Y., Watanabe, D., Ukibe, K., Inai, T., Ohtsu, I. Shimoi, H. and Takagi, H. (2012) Overexpression of the yeast transcription activator Msn2 confers furfural resistance and increases the initial fermentation rate in ethanol production. J. Biosci. Bioeng.113: 451-455 
  • Sasano, Y., Haitani, Y., Hashida, K., Ohtsu, I. Shima, J. and Takagi, H. (2012) Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker’s yeast. Microb. Cell Fact. 11: 40
  • Sasano, Y., Haitani, Y., Hashida, K., Ohtsu, I. Shima, J. and Takagi, H. (2012) Simultaneous accumulation of proline and trehalose in industrial baker’s yeast enhances fermentation ability in frozen dough. J. Biosci. Bioeng. 113: 592-595
  • Bach, T. M., Hibi, T., Nasuno, R., Matsuo, G., Sasano, Y. and Takagi, H. (2012) Production of N-acetyl cis-4-hydroxy-L-proline by the yeast N-acetyltransferase Mpr1. J. Biosci. Bioeng. 114: 160-165
  • 原島 俊  生物工学および生物工学関連―JABEE「生物工学および生物工学関連分野」の誕生―  JABEEのあゆみ―設立から13年(1999-2012)―、(社)日本技術者教育認定機構 広報・啓発委員会編集、(社)日本技術者教育認定機構発行、213-216 (2012)
  • 原島 俊  酵母における多様性創出ゲノム工学とその育種への応用―微生物育種工学のパラダイムシフト―、生物工学、Vol.90、No6、302-305 (2012)

2011

       
  • 原島 俊、シリーズ ゲノム解析、ゲノム改変操作にもとづく合成ゲノム生物学への展望―出芽酵母におけるゲノムのワードプロセシング技術の開発と応用、バイオサイエンスとインダストリー、Vol.69、No.4、279-83 (2011) 
  • Sugiyama, M., Nugroho, S., Iida, N., Sakai, T., Kaneko, Y., Harashima, S. (2011) Genetic interactions of ribosome maturation factors Yvh1 and Mrt4 influence mRNA decay, glycogen accumulation, and the expression of early meiotic genes in Saccharomyces cerevisiae. J. Biochem. 150(1):103-11.
  • Chuwattanakul, V., Kim, Y., Sugiyama, M., Nishiuchi, H. Miwa, H., Kaneko, Y., Harashima, S. (2011) Construction of a Saccharomyces cerevisiae strain with a high level of RNA. J. Biosci. Bioeng. 112(1):1-7.
  • Prasetyo, J., Naruse, K., Kato, T., Boonchird, C., Harashima S., Park, Y., E. (2011) Bio-conversion of paper sludge to biofuel by simultaneous saccharification and fermentation using a cellulase of paper sludge origin and thermotolerant Saccharomyces cerevisiae TJ14. Biotechnology for Biofuels 4:35.
  • Akao, T., Yashiro, I., Hosoyama, A., Kitagaki, H., Horikawa, H., Watanabe, D., Akada, R., Ando, Y., Harashima S., Inoue, T., Inoue, Y., Kajiwara, S., Kitamoto, K., Kitamoto, N., Kobayashi, O., Kuhara, S., Masubuchi, T., Mizoguchi, H., Nakao, Y., Nakazato A., Namise, S., Oba, T., Ogata, T., Ohta A., Sato, M., Shibasaki, S., Takatsume, Y., Tanimoto, S., Tsuboi, H., Yamashita, N., Yoda, K., Ishikawa, T., Iwashita, K., Fujita, N., Shimoi, H. (2011) Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no. 7. DNA Research 18(6):423-34.

2010

  • 杉山 峰崇, 片倉 啓雄, 金子 嘉信, 原島 俊 (2010) 特集 [グリーン材料とグリーンエネルギー], 高温耐性・酸耐性スーパー酵母による廃棄布類からのバイオエタノール生産. 繊維学会誌 (繊維と工業), Vol.66, No.5, 159-63.
  • Moukamnerd, Ch., Kino-oka, M., Sugiyama, M., Kaneko, Y., Boonchird, Ch., Harashima, S., Noda, H., Ninomiya, K., Shioya, S., Katakuka, Y. (2010) Ethanol Production from Biomass by Repetitive Solid-Sate Fed-Batch Fermentation with Continuous Recovery of Ethanol. Appl. Microbiol. Biotechnol. 88(1):87-94.
  • Hermansyah, Lavina, WA., Sugiyama, M., Kaneko, Y., Harashima, S. (2010) Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Δptp2Δmsg5 protein phosphatase double disruptant. Arch. Microbiol. 192(3):157-65.
  • Hirasaki, M., Nakamura, F., Yamagishi, K., Numamoto, M., Shimada, Y., Uehashi, K., Muta, S., Sugiyama, M., Kaneko, Y., Kuhara, S., Harashima, S. (2010) Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae. J. Biosci. Bioeng. 109(5):433-41.
  • Katakura, Y., Moukamnerd, C., Harashima, S., Kino-Oka, M. (2010) Strategy for preventing bacterial contamination by adding exogenous ethanol in solid-state semi-continuous bioethanol production. J. Biosci. Bioeng. 111(3):343-5.
  • Hirasaki, M., Horiguchi, M., Numamoto, M., Sugiyama, M., Kaneko, Y., Nogi, Y., Harashima, S. (2010) Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Hal5 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state. J. Biosci. Bioeng. 111(3):249-54.

2009

  • Hermansyah, Sugiyama, M., Kaneko, Y., Harashima, S. (2009) Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. Arch. Microbiol. 191(9):721-33.
  • Auesukaree, Ch., Damnernsawad, A., Kruatrachue, M., Pokethitiyook, P., Boonchird, Ch., Kaneko, Y., Harashima, S. (2009) Genome-wide identification of genes involved in tolerance to various environmental stresses in Saccharomyces cerevisiae. J. Appl. Genet. 50(3):301-10.
  • Sugiyama, M., Yamagishi, K., Kim, YH., Kaneko, Y., Nishizawa, M., Harashima, S. (2009) Advances in molecular methods to alter chromosomes and genome in the yeast Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 84(6):1045-52.
  • 藤田 清士・森本 健志・奥西 有理・池 道彦・原島 俊、大阪大学工学部・工学研究科の海外夏期英語研修への挑戦、生産と技術、Vol.61、No.4、101-104 (2009)

2008

  • Prasitchoke, P., Kaneko, Y., Bamba, T., Fukusaki, EI., Kobayashi, A., Harashima, S. (2008) The essential fatty acid myristate causes severe growth retardation in Hpelo disruptants of the yeast Hansenula polymorpha. Arch. Microbiol. 189(4):297-304.
  • Auesukaree, C., Fuchigami, I., Homma, T., Kaneko, Y., Harashima, S. (2008) Ddi1p and Rad23p play a cooperative role as negative regulators in the PHO pathway in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 25;365(4):821-5.
  • Hirasaki, M., Kaneko, Y., Harashima, S. (2008) Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae. Gene 15;409(1-2):34-43.
  • Yamagishi, K., Sugiyama, M., Kaneko, Y., Harashima, S. (2008) Conditional chromosome splitting in Saccharomyces cerevisiae using the homing endonuclease PI-SceI. Appl. Microbiol. Biotechnol. 79(4):699-706.
  • Sugiyama M, Nakazawa T, Murakami K, Sumiya T, Nakamura A, Kaneko Y, Nishizawa M, Harashima S. (2008) PCR-mediated one-step deletion of targeted chromosomal regions in haploid Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 80(3):545-53.
  • Yamagishi K, Sugiyama M, Kaneko Y, Harashima S. (2008) Conditional chromosome splitting in Saccharomyces cerevisiae using the homing endonuclease PI-SceI. Appl. Microbiol. Biotechnol. 79(4):699-706.
  • Yamagishi K, Sugiyama M, Kaneko Y, Nishizawa m, Harashima S. (2008) Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae. J. Biosci. Bioeng. 106(6):563-567.

2007

       
  • 杉山 峰崇・金 連姫・山岸 一雄・金子 嘉信・原島 俊、出芽酵母ゲノムの再構成技術と最適ゲノムファクトリー 微生物機能を活用した革新的生産技術の最前線―ミニマムゲノムファクトリーと―システムパイオロジー―、清水 昌・大竹 久夫・藤尾 達郎・穴澤 秀治編、シーエムシー出版、77-87 (2007)
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  • Prasitchoke, P., Kaneko, Y., Bamba, T., Fukusaki, E., Kobayashi, A. and Harashima, S.(2007) Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha. Gene 391:16-25.
  • Murakami, K., Tao, E., Ito, Y., Sugiyama, M., Kaneko, Y., Harashima, S., Sumiya, T., Nakamura, A. and Nishizawa, M. (2007) Large scale deletions in the Sacchraromyces cerevisiae genome create strains with altered regulation of carbon metabolism. Appl. Microbiol. Biotechnol. 75:589-597.
  • Prasitchoke, P., Kaneko, Y., Sugiyama, M., Bamba, T., Fukusaki, E., Kobayashi, A. and Harashima, S. (2007) Functional analysis of very long-chain fatty acid elongase gene, HpELO2, in the methylotrophic yeast Hansenula polymorpha. Appl. Microbiol. Biotechnol. 76:417-427.
  • Tamai, Y., Kanai, K. and Kaneko, Y. (2007) A structural and phylogenetic study of the HO gene from Saccharomyces bayanus var. uvarum. Biosci. Biotechnol. Biochem. 71:1850-1857.